[The influence of protoplast media on the tumor induction on kalanchoe leaves].

Experiments concerning tumor transformation of plant cells or protoplasts need complete knowledge of the effects of media components. Here inhibiting effects of cellulase Onozuka, glucose, sucrose and mannitol (up to 0.3M), of tissue culture media, and of the antibiotic aureomycin on the tumor initiation process in Kalanchoe leaves are reported. Also the influence of these substances on the growth of the tumor inducing Agrobacterium tumefaciens is studied. The results lead to the design of conditions which should be favourable to in vitro transformation.